| Unlockable | How to Unlock |
|---|
| Cao Cao | Clear Musou Mode with 3 Wei characters |
| Cao Pi | Deafeat Cao Pi within 5 minutes on Sun Jian side of He Fei |
| Cao Ren | Succeed in Battle Objective 2 on the Lu Bu side of Fan Castle |
| Diao Chan | Clear Musou Mode with 1 character from each Kingdom |
| Dong Zhuo | Succeed in Battle Objective 1 on the Lu Bu side of The Battle of Hu Lao Gate |
| Gan Ning | Clear Musou Mode with 1 Wu character |
| Guan Ping | Succeed in 2 battle objectives on the Shu side of Fan Castle and finish battle with Guan Ping alive |
| Huang Gai | Succeed in Battle Objective 3 on the Wu side of The Battle of Chi Bi |
| Huang Zhong | Succeed in 1 battle objective on the Wei side of Ding Jun Mountain |
| Ling Tong | Succeed in Battle Objective 1 on the Wei side of the Battle of Shi Ting |
| Liu Bei | Clear Musou Mode with 3 Shu characters |
| Lu Bu | Clear Musou Mode with Liu Bei, Cao Cao, Diao Chan, and Sun Jian |
| Lu Meng | Succeed in 2 battle objectives on the Wei side of He Fei |
| Ma Chao | Succeed in battle objective 2 on the Wei side of Han Zhong Attack Defense Battle |
| Pang Tong | Succeed in 3 battle objectives at Cheng Du battle and finish battle with Pang Tong alive |
| Sun Ce | Succeed in Battle Objective 1 on the Sun Jian side of the Battle of Xia Pi |
| Sun Jian | Clear Musou Mode with 3 Wu characters |
| Sun Quan | Succeed in Battle Objective 1 on the Lu Bu side of The Battle of Chi Bi |
| Taishi Ci | Defeat him in the Battle for Wu Territory |
| Wei Yan | Succeed in 2 battle objectives on the Shu side of WuZhang Plains and finish battle with Wei Yan alive |
| Xiahou Yuan | Succeed in 1 battle objective on the Shu side of Ding Jun Mountain |
| Xiao Quio | Succeed in Battle Objective 3 on the Wu side of the Battle of Shi Ting |
| Xu Huang | Succeed in 2 battle objectives on the Wei/Wu side of Fan Castle |
| Xu Zhu | He Fei, Wu side, Personally kill Cao Ren and Xu Chu |
| Yuan Shao | As Wei, complete all 3 targets and capture Wu Chao before completing the stage. |
| Yue Ying | Succeed in Battle Objective 1 on the Wei side of the Battle of Wu Zhang Plains |
| Zhang Jiao | Beat stage with completing all three targets in the Yellow Turban Rebellion. Playable in only Free Mode and Challenge Mode |
| Zhang Liao | Clear Musou Mode with 1 Wei character |
| Zhen Ji | Xu Du Invasion Battle, Lu Bu Scenario, succeed Battle Objective 1. |
| Zheng He | Succeed in 2 battle objectives on the Shu side of Han Zhong Attack Defense Battle |
| Zhou Tai | Succeed in 3 battle objectives on the Shu side of Yi Ling |
| Zhuge Liang | Clear Musou Mode with 1 Shu character |
